Journal: Blood

Article Title: Platelets can enhance vascular permeability.

doi: 10.1182/blood-2012-02-413047

Figure Lengend Snippet: Figure 4. Serotonin is present in the SF of patients with RA and can promote the gap formation in joint vasculature. (A) Serotonin concentrations were measured in SF of RA and OA patients using an ELISA serotonin kit (n 27). **P .0012. (B) Serotonin suffices to promote prompt formation of gaps in joints. Diluent (PBS) or serotonin (20 mg/kg) was injected intrave- nously in mice before administration of 0.45-m-diameter fluores- cent microspheres. The fluorescence was portrayed using an in vivo imaging system 45 minutes after the microsphere injection (left). The radiant efficiency quantifications in the ankle joints for control (diluent) and serotonin injections are presented on the right. ***P .0001; *P .024.

Article Snippet: The serotonin content in platelet lysates and in mouse whole blood was measured using serotonin ELISA Kit (Fitzgerald Industries International).

Techniques: Enzyme-linked Immunosorbent Assay, Injection, In Vivo Imaging, Control

Journal: Blood

Article Title: Platelets can enhance vascular permeability.

doi: 10.1182/blood-2012-02-413047

Figure Lengend Snippet: Figure 5. Mice deficient in platelet-derived serotonin have reduced gap formation. (A) Serotonin concentrations were measured in whole blood from mast cell-deficient mice (KitW-sh), neutrophil-depleted mice, platelet-depleted mice, and their corresponding control mice using an ELISA serotonin kit. (B) Serotonin concentration was measured in platelet isolated from WT and Slc6a4/ mice. (C) WT and Slc6a4/ arthritic mice were injected intravenously with 0.45-m-diameter fluorescent microsphere, and the fluorescence was visualized 5 minutes later using an in vivo imaging system. Control mice (WT nonarthritic) and WT and Slc6a4/ arthritic mice all received the same concentration of fluorescent microspheres. The radiant efficiency quantifications in the ankle joints for all mice are presented, and representative results are presented at right. ***P .004. (D) Mice (17/group) sufficient and deficient in SERT expression were injected with 125 L K/BxN serum at day 0 and day 2 and the signs of arthritis monitored daily. The mouse arthritis experiment is presented as mean SEM. P .01. (E) SERT pharmacologic inhibition was performed in C57BL/6J mice by treating the mice with fluoxetine for 21 days before administration of K/BxN serum. Arthritic (treated or not with fluoxetine) and control (nonarthritic) mice were injected intravenously with 0.45-m-diameter fluorescent microsphere, and the fluorescence was visualized 5 minutes later using an in vivo imaging system. The radiant efficiency quantifications in the ankle joints for all mice are presented, and representative results are presented at right. **P .0092. (F) Serotonin concentrations in platelets isolated from mice treated or not with fluoxetine were measured by ELISA.

Article Snippet: The serotonin content in platelet lysates and in mouse whole blood was measured using serotonin ELISA Kit (Fitzgerald Industries International).

Techniques: Derivative Assay, Control, Enzyme-linked Immunosorbent Assay, Concentration Assay, Isolation, Injection, In Vivo Imaging, Expressing, Inhibition

Journal: Journal of Microbiology and Biotechnology

Article Title: The Effect of Lactobacillus gasseri BNR17 on Postmenopausal Symptoms in Ovariectomized Rats

doi: 10.4014/jmb.2105.05032

Figure Lengend Snippet: Serum estradiol (E2) ( A ), Calcitonin ( B ), Osteocalcin ( C ), and 5-HT-2A ( D ) were measured after 14 weeks of initial administration ( n = 6~8 rats per group). Sophora japonica fruit extract (SJFE) treatment was used to create the positive control.

Article Snippet: The concentrations of estradiol (E2, CSB-E05110r), osteocalcin (CSB-E05129r), calcitonin (CSB-E05132r), estradiol (CSB-E05110r), and Serotonin 2A (5-HT-2A, CSB-E14956r) were assessed using an ELISA kit (CUSABIO, China).

Techniques: Positive Control

Journal: Life

Article Title: Increased Levels of Beta-Endorphin and Noradrenaline after a Brief High-Impact Multidimensional Rehabilitation Program in Multiple Sclerosis

doi: 10.3390/life12050755

Figure Lengend Snippet: β-endorphins, adrenaline, dopamine, noradrenaline, cortisol, and serotonin concentration (pg/mL) detected by ELISA in serum sample of MS patients at baseline (T0) and at the end of the B-HIPE (T1). The statistical analyses were assessed by Wilcoxon test using the MEDCALC statistical package. Median, interquartile range, and significant differences are shown.

Article Snippet: Serum concentrations of serotonin were determined by competitive enzyme immunoassay using a Human Serotonin ELISA Kit (R&D Systems) according to the manufacturer’s recommendations.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Cell

Article Title: Serotonin reduction in post-acute sequelae of viral infection

doi: 10.1016/j.cell.2023.09.013

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Serotonin ELISA kits , Novus biologicals , KA1894.

Techniques: Virus, Clinical Proteomics, Recombinant, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Derivatization, Single Cell, TaqMan Assay, Software, Imaging, Control, Electron Microscopy, Low Protein Binding, Membrane